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Background: Sleep deprivation is the restriction or at times, lack of sleep in an organism as result of lifestyle or environmental factors. It can affect the male reproductive system especially testicular function due to Inflammatory and oxidative stress mediators playing important roles.This study evaluated the ameliorative effect of honey on inflammatory and oxidative stress mediators in sleep deprived adult male wistar rats.
Methods and Material: Twenty-five rats were divided into five groups (n=5) and designated as follows: Group 1: Control, Group 2: sleep-deprived (SD), Group 3: sleep deprived and sleep recovery (SD+SR), Group 4: sleep-deprived honey treated (SD+HONEY), Group 5: sleep-deprived and recovery HONEY treated (SD+SR+HONEY). Rats were sleep deprived for five days, Honey 1ml/kg was given orally for honey treated groups while recovery groups were allowed to recover for five days. At the end of the experiments, cortisol, testosterone, interleukin 6 (IL-6), and c reactive protein (CRP) were analyzed while testicular malondialdehyde (MDA), catalase (CAT), and glutathione (GSH) were also evaluated. Data was expressed as means
± standard error of the mean (SEM). Statistical group analysis was performed with Graph pad (Prism 7) statistical software and statistically significant differences were accepted at p < 0.05.
Results: Rats in the SD group had significantly increased levels of MDA and cortisol, IL-6, and CRP levels (p<0.05), while showing significantly reduced levels of testosterone, GSH, and CAT (p<0.05). Treatment with honey reversed the changes in the MDA, GSH, CAT, cortisol, testosterone, IL 6, and CRP levels (p<0.05), while sleep recovery was also helpful in reversing the changes.
Conclusions: Honey administration ameliorated the high levels of testicular inflammatory and oxidative stress markers caused by sleep deprivation.


Honey Inflammation Oxidative stress Sleep deprivation Sleep recovery

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